Isolation, Identification and Quantification of Compounds with gastroprotective activity present in Centaurea solstitialis

  • Ricardo Enrique Toso UNLPam Facultad de Cs. Veterinarias
  • Mario Skliar UNS

Keywords:

Centaurea solstitialis

Abstract

Centaurea solstitialis is a plant with antiulcerous properties. To isolate and identificate bioactive compounds responsible for the gastroprotective activity, phytochemist bioguided studies were carried out in flavonoids related with this pharmacological activity. A methanolic fraction, obtained from dried heads of the plant, was extracted with ethyl acetate previous hydrolysis with hydrochloric acid. Ethyl acetate fraction was chromatografhied in thin layer on plates of silica gel and the bands, that comigrated with authentic standards of quercetin (Rf = 0.80) and kaempferol (Rf = 0.89), became purified by HPLC, getting the isolating of the two flavonoids. The compounds were recovered and identified by mass spectrometry. Quantitative essays allowed to determine that the heads of the plant contain 0.63 and 1.47 mg of quercetin and kaempferol per gram of dry matter respectively

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Author Biographies

Ricardo Enrique Toso, UNLPam Facultad de Cs. Veterinarias

Formación en Farmacología. Dirige el Centro de Invest. y Desarrollo de Fármacos (CIDEF) de la FCV-UNLPam donde participa en el desarrollo de trab. de invest .Desarrollo protocolos de extracción de compuestos vegetales y ensayos para determinar su bioactividad utilizando mod. in vivo e in vitro

Mario Skliar, UNS

Cátedra de Farmacognosia, Depto. de Biología, Bioquímica y Farmacia, Universidad  Nacional del Sur, San Juan 670, (8000) Bahía Blanca

Published

2017-09-22

How to Cite

Toso, R. E., & Skliar, M. (2017). Isolation, Identification and Quantification of Compounds with gastroprotective activity present in Centaurea solstitialis. Ciencia Veterinaria, 4(1), 17–27. Retrieved from https://ojs.unlpam.edu.ar/index.php/veterinaria/article/view/1963

Issue

Section

Artículos de Investigación