Production and potency of PMycobacterium phlei ans Mycobacterium fortuitum isolated soils from La Pampa, Argentina

  • Amelia Bernardelli SENASA
  • Delia Susana Oriani UNLPam. Facultad de Ciencias Veterinarias
  • Bernardo Alonso SENASA . Servicio Nacional de Sanidad y Calidad Agroalimentaria

Keywords:

Sensitines, Nontuberculous Mycobacteria, Protein Purified Derivative-PPD, Cross Reactions, Mycobacterium bovis

Abstract

In countries that have managed to eradicate bovine tuberculosis, has been shown to interfere with certain MNT tuberculosis diagnosis, when applied to the intradermal purified protein derivative Mycobacterium bovis PPD. It has also been established that some species can invade and replicate MNT inside Acanthamoeba parasites such as Dictyostelium discoideum, these protozoa may act as reservoirs of MNT mammals parasitized. Exist in mammals in the natural environment of lakes, sewage, soil, environmental dust,etc.,and manmade artificial enviroments such as drinking water, aquariums, plactonica and biofilms. The objectives of the proyect were to develop the sensitines (PPD) from the isolates of NTM isolated from soils of the province of La Pampa and check possible cross-reaction with PPD bovine.Two sensitines series of Mycobacterium fortuitum corresponding to and another Mycobacterium phlei, with protein concentration of 1.5 mg/mL. Guinea pigs were sensitized with Mycobacterum phlei, Mycobacterium fortuitum and Mycobacterium bovis power with sterile vaseline and pumice. After 120 days tests potency were carried out on the flanks of guinea pigs. The relative activity in guinea pigs sensitized with Mycobacterium bovis was of 2.18% with M.phlei and of 0.4% for the PPD Mycobacterium fortuitum ,wich shows that the environmental mycobacteria had no relevant actions in guinea pigs tuberculin intradermal reaction

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Author Biographies

Amelia Bernardelli, SENASA

SENASA ( DILAB)

Directora de  Bacteriología. Dirección Nacional de Diagnósticos, control y métodos

Delia Susana Oriani, UNLPam. Facultad de Ciencias Veterinarias

Trayectoria en investigación de Micobacterias ambientales, considerando distribución geográfica en molisoles, oxisoles, aridisoles y suelos lateriticos de la República Argentina. Identificación de micobacterias aisladas de humedales, agua de red, y agua de lluvia en estaciones climáticas extremas. Poder patógeno de las mismas en modelo animal de laboratorio. Valoración de diferentes desinfectantes. Desarrollo de modelo animal para la valoración de poder patógeno experimental de inoculantes vegetales. Colaboración en desarrollo de actividad antimicrobiana de extractos vegetale

Published

2017-07-31

How to Cite

Bernardelli, A., Oriani, D. S., & Alonso, B. (2017). Production and potency of PMycobacterium phlei ans Mycobacterium fortuitum isolated soils from La Pampa, Argentina. Ciencia Veterinaria, 14(1), 85–91. Retrieved from https://ojs.unlpam.edu.ar/index.php/veterinaria/article/view/1839

Issue

Section

Artículos de Investigación